UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G (Rule 13d-102) Information To Be Included In Statements Filed Pursuant To Section 240.13d-1(b), (c), and (d) and Amendments Thereto Filed Pursuant To Section 240.13d-2 Under the Securities Exchange Act of 1934 (Amendment No. 1)* Pressure BioSciences, Inc. (Name of Issuer) Common Stock, $0.01 par value per share (Title of Class of Securities) 74112E109 (CUSIP Number) December 31, 2012 (Date of Event Which Requires Filing of This Statement) Check the appropriate box to designate the rule pursuant to which this Schedule is filed: Rule 13d-1(b) X Rule 13d-1(c) Rule 13d-1(d) 1 NAMES OF REPORTING PERSONS Clayton A. Struve 2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP (SEE INSTRUCTIONS) (a) (b) X 3 SEC USE ONLY 4 CITIZENSHIP OR PLACE OF ORGANIZATION United States of America NUMBER OF SHARES BENEFICIALLY OWNED BY EACH REPORTING PERSON WITH 5 SOLE VOTING POWER 51,664 6 SHARED VOTING POWER 0 7 SOLE DISPOSITIVE POWER 51,664 8 SHARED DISPOSITIVE POWER 0 9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 51,664 10 CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES (SEE INSTRUCTIONS) 11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW 9* 0.4% 12 TYPE OF REPORTING PERSON (SEE INSTRUCTIONS) IN * Based on 11,694,889 shares of the Issuer's common stock issued and outstanding based on Amendment No. 1 to the Issuer's Quarterly Report on Form 10-Q/A, filed with the Securities and Exchange Commission on October 31 2012. 1 NAMES OF REPORTING PERSONS CSS, LLC 2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP (SEE INSTRUCTIONS) (a) (b) X 3 SEC USE ONLY 4 CITIZENSHIP OR PLACE OF ORGANIZATION United States of America NUMBER OF SHARES BENEFICIALLY OWNED BY EACH REPORTING PERSON WITH 5 SOLE VOTING POWER 401,935 6 SHARED VOTING POWER 0 7 SOLE DISPOSITIVE POWER 401,935 8 SHARED DISPOSITIVE POWER 0 9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 401,935 10 CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES (SEE INSTRUCTIONS) 11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW 9* 3.4% 12 TYPE OF REPORTING PERSON (SEE INSTRUCTIONS) BD Item 1(a) Name of Issuer: Pressure BioSciences, Inc. Item 1(b) Address of Issuer?s Principal Executive Offices: 14 Norfolk Avenue South Easton, MA 02375 Item 2(a) Name of Person Filing: Clayton A. Struve and CSS, LLC due to Clayton A. Struve's affiliation as a member. Item 2(b) Address of Principal Business Office or, if none, Residence: The address of the principal business office of each of the Reporting Persons is: 175 W. Jackson Blvd, Suite 440 Chicago, IL 60604 Item 2(c) Citizenship: United States. Item 2(d) Title of Class of Securities: Common Stock, $0.01 par value per share Item 2(e) CUSIP Number: 74112E109 Item 3 Not applicable. Item 4 Ownership. Provide the following information regarding the aggregate number and percentage of the class of securities of the issuer identified in Item 1. (a) Amount beneficially owned: See item 9 of cover pages. (b) Percent of class: See item 11 of cover pages. (c) Number of shares as to which the person has: (i) Sole power to vote or to direct the vote: (ii) Shared power to vote or to direct the vote: (iii) Sole power to dispose or to direct the disposition of: (iv) Shared power to dispose or to direct the disposition of: See items 5-8 of cover pages. Clayton A. Struve owns 51,664 shares of common stock of the issuer. 401,935 shares of common stock of the issuer are held by CSS, LLC where Clayton A. Struve is a member. Item 5 Ownership of Five Percent or Less of a Class. If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than 5 percent of the class of securities, check the following. X Item 6 Ownership of More than Five Percent on Behalf of Another Person. Not applicable. Item 7 Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company or Control Person. Not applicable. Item 8 Identification and Classification of Members of the Group. Not applicable. Item 9 Notice of Dissolution of Group. Not applicable. Item 10 Certification. By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect. SIGNATURE After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct. Date: February 8, 2013 Date: February 8, 2013 By: __/s/ Clayton A. Struve_______ Name: Clayton A. Struve CSS, LLC By: __/s/ Clayton A. Struve_______ Name: Clayton A. Struve * The remainder of this cover page shall be filled out for a reporting person?s initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter disclosures provided in a prior cover page. The information required on the remainder of this cover page shall not be deemed to be filed for the purpose of Section 18 of the Securities Exchange Act of 1934 (Act) or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes). ________________________ (continued...) (continued...) 5 Error! Unknown document property name. SCHEDULE 13G